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KMID : 0355620160420020090
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Journal of Korean Association of Oral and Maxillofacial Surgeons
2016 Volume.42 No. 2 p.90 ~ p.98
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Demineralized dentin matrix combined with recombinant human bone morphogenetic protein-2 in rabbit calvarial defects
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Um In-Woong
Hwang Suk-Hyun
Kim Young-Kyun
Kim Moon-Young
Jun Sang-Ho
Ryu Jae-Jun
Jang Hyon-Seok
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Abstract
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Objectives: The aim of this study was to compare the osteogenic effects of demineralized dentin matrix (DDM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in rabbit calvarial defects with DDM and anorganic bovine bone (ABB) combined with rhBMP-2.
Materials and Methods: Four round defects with 8-mm diameters were created in each rabbit calvaria. Each defect was treated with one of the following: 1) DDM, 2) ABB/rhBMP-2, or 3) DDM/rhBMP-2. The rhBMP-2 was combined with DDM and ABB according to a stepwise dry and dip lyophilizing protocol. Histological and microcomputed tomography (?CT) analyses were performed to measure the amount of bone formation and bone volume after 2- and 8-week healing intervals.
Results: Upon histological observation at two weeks, the DDM and ABB/rhBMP-2 groups showed osteoconductive bone formation, while the DDM/rhBMP-2 group showed osteoconductive and osteoinductive bone formation. New bone formation was higher in DDM/rhBMP-2, DDM and ABB decreasing order. The amounts of bone formation were very similar at two weeks; however, at eight weeks, the DDM/rhBMP-2 group showed a two-fold greater amount of bone formation compared to the DDM and ABB/rhBMP-2 groups. The ?CT analysis showed markedly increased bone volume in the DDM/rhBMP-2 group at eight weeks compared with that of the DDM group. Notably, there was a slight decrease in bone volume in the ABB/rhBMP-2 group at eight weeks. There were no significant differences among the DDM, ABB/rhBMP-2, and DDM/rhBMP-2 groups at two or eight weeks.
Conclusion: Within the limitations of this study, DDM appears to be a suitable carrier for rhBMP-2 in orthotopic sites.
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KEYWORD
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Demineralized dentin matrix, Recombinant human bone morphogenetic protein-2, Microcomputed tomography, Histomorphometric analysis
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